hsv 1 wt strain kos Search Results


94
ATCC hsv1 strain kos atcc vr 1493d
Comparison of Phusion vs Q5 DNA Polymerases for amplification of <t>HSV1.</t> Amplification of serially diluted HSV1 (5.23 × 10 5 to 5.23 ×10 2 copies/reaction) in triplicates with (A) Phusion-LOD of 5.23 × 10 4 copies and with (B) Q5-LOD of 5.23 × 10 3 copies.
Hsv1 Strain Kos Atcc Vr 1493d, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsv+1+wt+strain+kos/Human+herpesvirus+1+DNA%3B+HSV-1+strain+KOS/pmc11175599-86-16-18
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hsv1 strain kos atcc vr 1493d - by Bioz Stars, 2026-09
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90
China Center for Type Culture Collection hsv-1 (kos strain)
Comparison of Phusion vs Q5 DNA Polymerases for amplification of <t>HSV1.</t> Amplification of serially diluted HSV1 (5.23 × 10 5 to 5.23 ×10 2 copies/reaction) in triplicates with (A) Phusion-LOD of 5.23 × 10 4 copies and with (B) Q5-LOD of 5.23 × 10 3 copies.
Hsv 1 (Kos Strain), supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsv+1+wt+strain+kos/hsv+1++kos+strain+/pm32474700-239-7-10
Average 90 stars, based on 1 article reviews
hsv-1 (kos strain) - by Bioz Stars, 2026-09
90/100 stars
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90
Medical Diagnostic Laboratories LLC hsv-1 kos strain
Comparison of Phusion vs Q5 DNA Polymerases for amplification of <t>HSV1.</t> Amplification of serially diluted HSV1 (5.23 × 10 5 to 5.23 ×10 2 copies/reaction) in triplicates with (A) Phusion-LOD of 5.23 × 10 4 copies and with (B) Q5-LOD of 5.23 × 10 3 copies.
Hsv 1 Kos Strain, supplied by Medical Diagnostic Laboratories LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsv+1+wt+strain+kos/hsv+1+kos+strain/pm19759281-23-7-20
Average 90 stars, based on 1 article reviews
hsv-1 kos strain - by Bioz Stars, 2026-09
90/100 stars
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Comparison of Phusion vs Q5 DNA Polymerases for amplification of HSV1. Amplification of serially diluted HSV1 (5.23 × 10 5 to 5.23 ×10 2 copies/reaction) in triplicates with (A) Phusion-LOD of 5.23 × 10 4 copies and with (B) Q5-LOD of 5.23 × 10 3 copies.

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Comparison of Phusion vs Q5 DNA Polymerases for amplification of HSV1. Amplification of serially diluted HSV1 (5.23 × 10 5 to 5.23 ×10 2 copies/reaction) in triplicates with (A) Phusion-LOD of 5.23 × 10 4 copies and with (B) Q5-LOD of 5.23 × 10 3 copies.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Comparison, Amplification

Optimization of annealing temperature in HSV1. Temperature gradient experiments were performed for HSV1 in duplicates from 72 °C to 67 °C (rounded off) for a 10× dilution series ranging from 5.23×10 4 to 5.23 × 10 2 genome copies/reaction. As in the case of HCMV, product concentration decreases with decrease in temperature. ~72 °C was thus chosen as the optimum annealing temperature.

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Optimization of annealing temperature in HSV1. Temperature gradient experiments were performed for HSV1 in duplicates from 72 °C to 67 °C (rounded off) for a 10× dilution series ranging from 5.23×10 4 to 5.23 × 10 2 genome copies/reaction. As in the case of HCMV, product concentration decreases with decrease in temperature. ~72 °C was thus chosen as the optimum annealing temperature.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Concentration Assay

Standard curves for HCMV, HSV1 and HSV2. The quantification region (LOQ) is marked with a dashed line while the plateau region at lower concentrations is due to primer dimers. The serial dilution concentrations are as follows: HCMV-8.45 × 10 7 to 8.45×10 0 copies/reaction, HSV1– 5.23 × 10 7 to 5.23 × 10 0 copies/reaction and HSV2– 3.45 × 10 6 to 3.45 × 10 0 copies/reaction.

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Standard curves for HCMV, HSV1 and HSV2. The quantification region (LOQ) is marked with a dashed line while the plateau region at lower concentrations is due to primer dimers. The serial dilution concentrations are as follows: HCMV-8.45 × 10 7 to 8.45×10 0 copies/reaction, HSV1– 5.23 × 10 7 to 5.23 × 10 0 copies/reaction and HSV2– 3.45 × 10 6 to 3.45 × 10 0 copies/reaction.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Serial Dilution

Characteristics of the viral assay for HCMV,  HSV1  and HSV2 amplification.

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Characteristics of the viral assay for HCMV, HSV1 and HSV2 amplification.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Amplification

Inter and intra-assay variation for the three viruses.

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Inter and intra-assay variation for the three viruses.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Intra Assay, Inter Assay